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The medical relevance of methods to sample indoor air microbial pollution

Rylander R., Beijer L., 2003
health | microbiology | sampling
Bibliographic info: Healthy Buildings 2003 - Proceedings 7th International Conference (7th-11th December 2003) - National University of Singapore - Vol. 1., pp 711-714, 3 Tab., 10 Ref.
Languages: English

The aim of the investigation was to evaluate different methods to sample microbial cell wallagents (MCWA) indoors and to relate the results to clinical markers of inflammation amongpersons (n = 27), living in houses with suspected mould problems. Airborne particles weresampled for 8 h or by agitating floor dust. Sedimented particles were collected from floor dust.Analysis was done for endotoxin and (1?3)--D-glucan. Endotoxin was found only in a few ofthe agitated floor dust samples. There was a relation between airborne endotoxin levels and thesecretion of certain Th1-type cytokines from stimulated blood mononuclear cells and between(1?3)--D-glucan in floor dust and the total amount of IgE in serum. Boarder values were definedfor endotoxin at 0.2 ng/m3 and for (1?3)--D-glucan at 25 ng/mg floor dust.


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